AUTHOR=Borghi Maria Orietta , Bombaci Mauro , Bodio Caterina , Lonati Paola Adele , Gobbini Andrea , Lorenzo Mariangela , Torresani Erminio , Dubini Antonella , Bulgarelli Ilaria , Solari Francesca , Pregnolato Francesca , Bandera Alessandra , Gori Andrea , Parati Gianfranco , Abrignani Sergio , Grifantini Renata , Meroni Pier Luigi 

TITLE=Anti-Phospholipid Antibodies and Coronavirus Disease 2019: Vaccination Does Not Trigger Early Autoantibody Production in Healthcare Workers

JOURNAL=Frontiers in Immunology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.930074

DOI=10.3389/fimmu.2022.930074

ISSN=1664-3224

ABSTRACT=A molecular mimicry between SARS-CoV-2 and human proteins supports the possibility that autoimmunity takes place during Coronavirus disease 19 (COVID-19) contributing to tissue damage.  For example, anti-phospholipid antibodies (aPL) have been reported in COVID-19 as a result of such mimicry, and thought to contribute to the immunothrombosis characteristic of the disease. Consistently, active immunization with the virus spike protein may elicit the production of cross-reactive autoantibodies, including aPL. We prospectively looked at the aPL production in healthcare workers vaccinated with RNA- (BNT162b2, n. 100) or adenovirus-based vaccines (ChAdOx1, n. 50). Anti-cardiolipin, anti-beta2 glycoprotein I, anti-phosphatidylserine/prothrombin IgG, IgA, IgM before and after vaccination were investigated.  Anti-platelet factor 4 immunoglobulins were also investigated as autoantibodies associated with COVID-19 vaccination. Additional organ (anti-thyroid) and non-organ (anti-nuclear) autoantibodies and IgG against human proteome were tested as further post-vaccination autoimmunity markers. The antibodies were tested one or three months after the first injection of ChAdOx1 and BNT162b2, respectively; a 12-month clinical follow-up was also performed. Vaccination occasionally induced low titers of aPL and other autoantibodies but did not affect the titer of pre-existing autoantibodies. Few reactivities against a microarray of approximately 20,000 human proteins were found in the same cohort. Consistently, we did not record any clinical manifestation theoretically associated with an underlying autoimmune disorder. The data obtained after the vaccination (two doses for the RNA- and one dose for the adenovirus-based vaccines), and the clinical follow-up are not supporting the occurrence of an early autoimmune response in this cohort of healthcare workers.