AUTHOR=Fan Wenqi , Pang Haipeng , Shi Xiajie , Li Jiaqi , Wang Yimeng , Luo Shuoming , Lin Jian , Yu Haibo , Xiao Yang , Li Xia , Huang Gan , Xie Zhiguo , Zhou Zhiguang 

TITLE=Plasma-derived exosomal mRNA profiles associated with type 1 diabetes mellitus

JOURNAL=Frontiers in Immunology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.995610

DOI=10.3389/fimmu.2022.995610

ISSN=1664-3224

ABSTRACT=Backgrounds: Exosomes carry various types of transcripts such as messenger RNAs (mRNAs) and play an important role in mediating cell-to-cell communication, thus influencing multiple physiological and pathological processes. However, the role of exosomal mRNAs in T1DM are largely unknown. Here, we aimed to identify the plasma-derived exosomal mRNA expression profiles in T1DM and explore their potential biological function in T1DM. Materia and Methods: Plasma-derived exosomes were isolated from 10 patients with T1DM and 10 age-, sex- matched control subjects by size exclusion chromatography methods. Transmission electron microscopy, nanoparticle tracking analysis, and western blot analysis confirmed the presence of exosomes. The exosomal mRNAs were analyzed using Illumina Hiseq platform. Six differentially expressed mRNAs (DEMs) were randomly selected to determine the expression level by quantitative real-time PCR (qRT-PCR) in a larger cohort (T1DM subjects N=40; Control subject N=40). The biological functions of DEMs were predicted by Geno Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The protein-protein interaction networks were constructed to explore the potential associations among DEMs. Results: Totally, 112 DEMs were identified in T1DM, among which 66 mRNAs were up-regulated and 46 mRNAs were down-regulated. Four of six candidate exosomal mRNAs were successfully validated by qRT-PCR. Bioinformatics analysis indicated that these mRNAs were most significantly involved in positive regulation by host of viral transcription (GO enrichment analysis) and oxidative phosphorylation (KEGG pathway analysis). Conclusions: Our study reported the plasma-derived exosomal mRNAs expression profile of T1DM for the first time. Identified DEMs might be associated with the pathogenesis of T1DM and some DEMs have the potential to serve as biomarker and therapeutic target for T1DM.