AUTHOR=Gómez-Morón Álvaro , Requena Silvia , Pertusa Clara , Lozano-Prieto Marta , Calzada-Fraile Diego , Scagnetti Camila , Sánchez-García Inés , Calero-García Ana Adela , Izquierdo Manuel , Martín-Cófreces Noa B. 

TITLE=End-binding protein 1 regulates the metabolic fate of CD4+ T lymphoblasts and Jurkat T cells and the organization of the mitochondrial network

JOURNAL=Frontiers in Immunology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1197289

DOI=10.3389/fimmu.2023.1197289

ISSN=1664-3224

ABSTRACT=The organization of the mitochondrial network is relevant for the metabolic fate of T cells and their ability to respond to TCR stimulation. This arrangement depends on cytoskeleton dynamics in response to TCR and CD28 activation, which allows the polarization of the mitochondria through their change in shape, and their movement along the microtubules towards the immune synapse. This work focus on the role of Endbinding protein 1 (EB1), a protein that regulates tubulin polymerization and has been previously identified as a regulator of intracellular transport of CD3-enriched vesicles. By unifying the organization of the tubulin cytoskeleton and mitochondria during CD4 + T cell activation, this work highlights the importance of this connection for critical cell asymmetry together with metabolic functions such as glycolysis, mitochondria respiration, and cell viability. EB1-interferred cells show defective metabolic strength in activated T cells, pointing to a relevant connection of cytoskeleton and metabolism in response to TCR stimulation.