AUTHOR=Olaloye Oluwabunmi , Eke Chino , Jolteus Abigail , Konnikova Liza 

TITLE=Single cell analysis via mass cytometry of spontaneous intestinal perforation reveals alterations in small intestinal innate and adaptive mucosal immunity

JOURNAL=Frontiers in Immunology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.995558

DOI=10.3389/fimmu.2023.995558

ISSN=1664-3224

ABSTRACT=Spontaneous intestinal perforation (SIP) is a poorly understood severe gastrointestinal complications of prematurity. Using mass cytometry time of flight (CyTOF) of small intestinal tissue from neonates with SIP compared to second-trimester fetal and full-term neonatal tissue, we demonstrate immune dysregulation in SIP. SIP mucosa had an expansion of ckit+ neutrophils, an influx of naïve CD4 and CD8 T cells, and a reduction of effector memory T cells. SIP T cells were characterized by reduced CCR6 and CXCR3 expression and increased interferon-gamma (IFNg) expression after stimulation. These findings suggest that previously unrecognized immune dysregulation is associated with SIP and should be explored in future studies.