AUTHOR=Galloway Darrell R. , Li Jiahui , Nguyen Nguyen X. , Falkenberg Frank W. , Henning Lisa , Krile Robert , Chou Ying-Liang , Herron James N. , Hale J. Scott , Williamson E. Diane 

TITLE=Co-formulation of the rF1V plague vaccine with depot-formulated cytokines enhances immunogenicity and efficacy to elicit protective responses against aerosol challenge in mice

JOURNAL=Frontiers in Immunology

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1277526

DOI=10.3389/fimmu.2024.1277526

ISSN=1664-3224

ABSTRACT=Contribution to the field statement This manuscript describes a unique study which employed the use of depot-formulated cytokines to significantly enhance the immune response to the rF1V plague vaccine resulting in complete protection from a substantial aerosol challenge in a murine model. Our results provide additional evidence that depot-formulated cytokines offer a new approach toward vaccine enhancement. We have recently published that depot-formulated cytokines when formulated with the rF1V antigen result in significant induction of the germinal center (GC) Tfh reactions which induce long-term immunological memory to the rF1V antigen. In this study we report that the rF1V cytokine-formulated vaccine confers complete protection against aerosol challenge. Our results support and extend a series of studies which indicate the use of depot-formulated cytokines in vaccines constitute an emerging area of interest in understanding the basis of antigen presentation and the ability to induce long-term immune memory responses.