AUTHOR=Liu Yue , Bai Yulin , Chen Sijing , Pu Fei , Li Yaxian , Chi Hongshu , Zheng Zaiyu , Xu Peng , Zhou Tao 

TITLE=Molecular characterization, expression pattern and immunologic function of CD82a in large yellow croaker (Larimichthys crocea)

JOURNAL=Frontiers in Immunology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1301877

DOI=10.3389/fimmu.2024.1301877

ISSN=1664-3224

ABSTRACT=<p>Visceral white spot disease (VWND) caused by <italic>Pseudomonas plecoglossicida</italic> poses a major threat to the sustainable development of large yellow croaker (<italic>Larimichthys crocea</italic>) aquaculture. Genome-wide association analysis (GWAS) and RNA-seq research indicated that <italic>LcCD82a</italic> play an important role in resistance to visceral white spot disease in <italic>L. crocea</italic>, but the molecular mechanism of <italic>LcCD82a</italic> response to <italic>P. plecoglossicida</italic> infection is still unclear. In this study, we cloned and validated the Open Reading Frame (ORF) sequence of <italic>LcCD82a</italic> and explored the expression profile of <italic>LcCD82a</italic> in various tissues of <italic>L.crocea</italic>. In addition, two different transcript variants (<italic>LcCD82a-L</italic> and <italic>LcCD82a-S</italic>) of <italic>LcCD82a</italic> were identified that exhibit alternative splicing patterns after <italic>P. plecoglossicida</italic> infection, which may be closely related to the immune regulation during pathogenetic process of VWND. In order to explore the function of <italic>LcCD82a</italic>, we purified the recombinant protein of <italic>LcCD82a-L</italic> and <italic>LcCD82a-S</italic>. The bacterial agglutination and apoptosis function analysis showed that <italic>LcCD82a</italic> may involve in extracellular bacterial recognition, agglutination, and at the same time participate in the process of antigen presentation and induction of cell apoptosis. Collectively, our studies demonstrate that <italic>LcCD82a</italic> plays a crucial role in regulating apoptosis and antimicrobial immunity.</p>