AUTHOR=Wang Tao , Hao Jianfeng , Li Bing , Hyraht Ahjol , Wang Jialing , Xia Henglei , Wu Qingbin , Gao Wei , Chen Congxia , Yu Chuanqing , Gong Xiuqun , Li Ting , Zhang Mei , Xie Yinghai , Tao Xinrong 

TITLE=Single-cell and spatial transcriptomics reveal a stress-induced EMT-like epithelial subset driving immune activation in silica-injured lung

JOURNAL=Frontiers in Immunology

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1609616

DOI=10.3389/fimmu.2025.1609616

ISSN=1664-3224

ABSTRACT=The mechanism that lung epithelial cells regulate immune responses during chronic injury still remains unclear. Here, we combined single-cell RNA sequencing with spatial transcriptomics to track epithelial dynamics in silica (SiO2)-exposed mouse lungs. By day 56, SiO2 induced significant epithelial proliferation, followed with a distinct C0 subset emerging as the dominant population. C0 cells co-expressed surfactant genes (Sftpc, Scgb3a2), mesenchymal markers (Vim, Mmp12), and pro-inflammatory cytokines (Ccl6, S100a8/a9), reflecting a hybrid phenotype. Spatial and cell-cell interaction analyses showed C0 cells engaging macrophages and neutrophils through SPP1-CD44, APP-CD74, and GRN-MARCO signaling, driving immune recruitment and activation. Pseudotime and CytoTRACE analyses indicated that C0 cells represent a late-stage, low-stemness state with epithelial-mesenchymal transition (EMT)-like features. Taken together, these findings reveal a novel, stress-induced epithelial subset that amplifies immune crosstalk and tissue remodeling, offering new perspectives on silica-induced lung injury.