AUTHOR=Li Tianlong , Peng Dingkun , Yao Meng , Li Meilin , Wang Yijing , Li Su , Zhang Ding , Yang Bo , Qiu Hua-Ji , Li Lian-Feng 

TITLE=Immune organoids: emerging platforms for modeling and analyzing human adaptive immunity

JOURNAL=Frontiers in Immunology

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1632117

DOI=10.3389/fimmu.2025.1632117

ISSN=1664-3224

ABSTRACT=The rapid advancement of vaccines and immunotherapies has significantly improved public health. However, a significant translational gap remains between basic research and clinical application, largely attributed to the disconnect between in vitro studies and in vivo models. To bridge this gap, in vitro models of immune organs, including bone marrow, thymus, spleen, lymph nodes, and tonsils, have emerged as a promising solution. By integrating cutting-edge technologies such as ex vivo culture, microfluidic chips, engineered tissues, and organoid models, researchers have successfully established a new-generation in vitro immune simulation platform. This review systematically summarizes recent progress in immune organ-based in vitro models, outlines the current technological landscape and highlights the unique advantages of immune organoids within this field. Notably, we classify immune organoids into strictly and broadly defined categories based on their origin and construction methodology, while emphasizing the importance of multi-model integration. This platform provides a novel framework for advancing translational immunology research, particularly in the fields of adaptive immunity and vaccine development.