AUTHOR=Liu Songtao , Fang Yingying , Liu Ying , Li Xin , Sun Fei , Wu Yanling , Ma Zhen , Ma He 

TITLE=Effects of different LED spectra on growth and expression of GH/IGF-I axis and apoptosis related genes in juvenile Takifugu rubripes

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.1008068

DOI=10.3389/fmars.2022.1008068

ISSN=2296-7745

ABSTRACT=Light has long been known to have a profound influence on the growth and development of fish. The previous studies showed that different spectra had different effects on the growth of juvenile Takifugu rubripes. Among them, green light can promote the growth of Takifugu rubripes, but the influence mechanism is unknown. In this study, how different LED spectrum affect fish growth from the perspective of GH/IGF-I axis-related genes and apoptosis genes deeply explored. The effects of light spectrum on growth, melatonin synthesis, GH/IGF-I axis and relative expressions of apoptosis-related genes in juvenile Takifugu rubripes were studied, including arylalkylamine N-acetyltransferase (AANAT2), growth hormone (GH), growth hormone receptor type 1 (GHR1), growth hormone releasing hormone (GHRH), insulin-like growth factor type I (IGF-I), insulin-like growth factor type Ⅱ (IGF-Ⅱ), insulin-like growth factor binding protein (IGFBP), Bcl-2 protein family (Bcl-2), tumor suppressor (p53) and cysteine protease family (caspase 3, caspase 8, caspase 10) genes. The results showed that the growth rate was the highest in the green light group and the lowest in the yellow light group. The green light indeed promote the growth of Takifugu rubripes. The GHR1, IGF-I, IGF-Ⅱ and IGFBP of juvenile Takifugu rubripes cultured under green light were significantly higher than those of white (control group), blue and yellow light groups. The relative expression levels of GH and GHRH genes had no difference from those in the white light control group, and the relative expression levels of apoptosis genes in the green light group were significantly lower than those in the blue light and yellow light groups. Under red light, the relative expression levels of all growth genes on the GH/IGF-I growth axis were relatively high. But at the same time, the relative expression of caspase 10 gene in juvenile Takifugu rubripes was high, and the growth state was inhibited. It is speculated that red light might disturb the endocrine system of the juvenile Takifugu rubripes, thus impeded its growth and development. Therefore, different LED spectra can affect the growth of juvenile Takifugu rubripes by affecting the expressions of GH/IGF-I growth axis and apoptosis-related genes.