AUTHOR=Fu Jinxing , He Yanbin , Peng Chao , Tang Tianle , Jin Aihua , Liao Yanling , Shi Qiong , Gao Bingmiao 

TITLE=Transcriptome Sequencing of the Pale Anemones (Exaiptasia diaphana) Revealed Functional Peptide Gene Resources of Sea Anemone

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.856501

DOI=10.3389/fmars.2022.856501

ISSN=2296-7745

ABSTRACT=Sea anemone venom contains a complex and diverse arsenal of peptides and proteins of pharmacological and biotechnological interest, however, only venom from a few species has been explored from a global perspective to date. The primary objective of this study was to realize large-scale discovery of protein and peptide toxin sequences from different developmental stages of the sea anemone Exaiptasia diaphana. Using high-throughput transcriptome sequencing, we identified 533 putative protein and peptide toxin sequences that belong to 75 known superfamilies, in which most of the protein components corresponded to metalloproteases, pancreatic lipase-related protein like, chymotrypsinogen like, collagen, and G-protein coupled receptor, and the peptide components corresponded to ShK domain, Kunitz-type, thrombin, defensin, and insulin-like peptide. This study therefore provides a valuable high-throughput method to obtain the resources of sea anemone proteins and peptides, and lays a foundation for further targeted research on the diversity and pharmacological effects of sea anemone toxins.