AUTHOR=Masanja Fortunatus , Xu Yang , He Guixiang , Liang Feilong , Liu Xiaolong , Yang Ke , Mkuye Robert , Liang Jian , Deng Yuewen , Zhao Liqiang 

TITLE=Exploring HSP90 as a Biomarker for Marine Heatwaves in Pinctada maxima

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.913920

DOI=10.3389/fmars.2022.913920

ISSN=2296-7745

ABSTRACT=Many species, particularly marine organisms, are becoming more vulnerable to marine heatwaves as a result of climate change. Marine species are anticipated to perish during marine heatwaves, but there is a growing interest in learning why some can resist. Using Pinctada maxima as a model organism, we were able to clone a full-length cDNA encoding HSP90 with a deduced open reading frame of 2031 amino acid residues and a predicted molecular mass of 78.08 kD to understand better the effects of marine heatwaves on HSP90 gene expression in pearl oysters. The amino acid sequence of P. maxima HSP90 showed a high similarity with the Pinctada martensii HSP90 families. At 32°C and 36°C, the expression of PmHSP90 was significantly expressed and reached its highest level at 6 h, indicating that pearl oysters quickly up-regulated the expression of HSP90 in response to acute MHWs. Pearl oysters' temperature response was relieved, as best demonstrated by the dramatically reduced expression levels of PmHSP90 in the frequently reoccurring MHWs scenario. Using these findings, it is possible to predict acute and repeated MHWs in pearl oysters using P. maxima HSP90 as a molecular biomarker.