AUTHOR=Sun Yu , Guo Kefan , Yu Xiaobo , Li Yanhong , Yao Weizhi , Wu Zhengli 

TITLE=Molecular and biochemical effects on metabolism and immunity of Hyriopsis cumingii fed with four different microalgae

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2022.970781

DOI=10.3389/fmars.2022.970781

ISSN=2296-7745

ABSTRACT=Hyriopsis cumingii has attracted attention because of its pearl performance and water purification capacity. Realizing sustainable industrialized culture of H. cumingii or applying to bivalve biomanipulation for controlling water eutrophication needs urgent researches about the selection of suitable algae and effects of different microalgae on mussel physiology. To contrast molecular and biochemical effects of high-quality microalgae diets (Chlorella vulgaris, Navicula pelliculosa and Cyclotella sp.) with toxic Microcystis aeruginosa on metabolism and immune physiology of H. cumingii, levels of related enzymes and genes were analyzed during 28-day exposure period. Results showed that the Cyclotella sp. diet could significantly (P < 0.05) maintain higher levels of metabolic enzymes (GOT, GPT, PK, HK) and genes (CPT1, LDLR). C. vulgaris and N. pelliculosa treatments significantly (P < 0.05) reduced activities of these metabolic parameters. The M. aeruginosa treatment significantly (P < 0.05) enhanced levels of immune enzymes (AKP, SOD, CAT) and genes (HcIL-17, IAP) on day 1 or 7, and there was a significant (P < 0.05) reduction on day 28. Results suggested Cyclotella sp. was the suitable algae for H. cumingii, followed by C. vulgaris and N. pelliculosa, and toxic algae caused metabolic disorders, immune injury, and poor physiological status. The study has practical significance in the sustainable cultivation of H. cumingii and provides theoretical basis for bivalve biomanipulation in eutrophic water.