AUTHOR=Tian Kuo , Li Qi , Zhang Xiumei , Guo Haoyu , Wang Yihang , Cao Pinglin , Xu Shengyong , Li Weiye 

TITLE=Analysis of the expression and function of the CBL-CIPK network and MAPK cascade genes in Kandelia obovata seedlings under cold stress

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 10 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2023.1113278

DOI=10.3389/fmars.2023.1113278

ISSN=2296-7745

ABSTRACT=Mangroves are an important component of coastal wetland ecosystems, and low temperature is the main factor that limits its extension to higher latitudes. Kandelia obovata as one of the most cold-tolerant species in mangrove ecosystems can provide basis for the northward migration of mangrove ecosystems. In this study, K. obovata seedlings from Zhoushan (Zhoushan, Zhejiang, China) were selected for comparison of transcriptome changes in the roots, stems, and leaves before and after cold snaps to reveal the molecular mechanism of frost resistance. A total of 1560, 370, and 416 genes were differentially expressed in the roots, stems, and leaves before and after cold snaps, respectively. Among these differentially expressed genes, 13 positive and negative regulators were attributed to the CBL-CIPK signaling network and MAPK cascade, which might be related to the frost resistance mechanism of K. obovata. Transcription factors such as AP2/EREBP and bHLH were involved in regulating the synthesis pathways of ethylene, cytokinin, growth hormone, and flavonoids. Results provide new insights into the frost resistance mechanism of K. obovata seedlings.