AUTHOR=Shikina Shinya , Lin Tzu-Chieh , Chu Yu-Ling , Cheng Yin-Chu , Chang Yu-En , Wada Naohisa , Tang Sen-Lin , Iizuka Yoshiyuki , Chiu Yi-Ling 

TITLE=Culturing reef-building corals on a laboratory dish: a simple experimental platform for stony corals

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 10 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2023.1149495

DOI=10.3389/fmars.2023.1149495

ISSN=2296-7745

ABSTRACT=Development and maintenance of coral reef ecosystems rely on daily micro-biological activities of healthy corals. Although a comprehensive understanding of coral biological properties, as well as factors negatively affecting coral growth, are essential to conserve existing corals, the current lack of a tractable culture and experimental platform has delayed acquisition of such knowledge. Here we show a highly versatile culture system, "coral-on-a-laboratory dish" (CLD), allowing long-term culturing of various corals in plastic/glass Petri dishes with maintenance of their biological properties. Under optimized conditions, coral microcolonies (~5 mm x 5 mm fragments) of Pocillopora damicornis were cultured for several months in dishes with high survivorship and characteristic growth. These microcolonies maintained their biological properties, such as reproduction, skeleton formation, coralalgal symbiosis, ingestion, and digestion. Thus far, CLD has been used to maintain at least 4 other coral species from 4 other families, including the Acroporidae, the Pocilloporidae, the Poritidae, and the Merulinidae for more than 2 months with 100% survivorship. CLD is applicable to a variety of biophysiological studies, including coral-algal symbiosis and impact assessment of marine pollutants, e.g., a sunscreen substance, oxybenzone, and an organic biocide, Irgarol 1051, at cellular-level resolution. The CLD platform, which allows easy and inexpensive coral maintenance in a laboratory incubator, represents a technological breakthrough that will greatly advance coral studies.