AUTHOR=Li Jinbao , Wang Zhen , Cao Xiufei , Wang Jianmin , Gong Ye , Wang Xiuneng , Lai Wencong , Bu Xianyong , Zheng Jichang , Mai Kangsen , Ai Qinghui 

TITLE=Effects of supplemental mixed bile acids on growth performance, body composition, digestive enzyme activities, skin color, and flesh quality of juvenile large yellow croaker (Larimichthys crocea) in soybean oil based diet

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 10 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2023.1149887

DOI=10.3389/fmars.2023.1149887

ISSN=2296-7745

ABSTRACT=A 10-week feeding trial was conducted to investigate the effects of dietary mixed bile acids (BA) on growth performance, body composition, digestive enzyme activities, skin color and flesh quality of juvenile large yellow croaker (initial weight, 13.10 ± 0.18 g). Four iso-nitrogenous and iso-lipidic experimental diets were formulated and designated as soybean oil (SO), SO supplemented with 300 (BA300), 600 (BA600), and 1200 (BA1200) mg/kg bile acids. Results showed that specific growth rate presented quadratic pattern with supplemental bile acids level, peaking at BA600 group. Meanwhile, feed conversion rate of cultured fish was significantly improved in BA600 group (P < 0.05). The redness (a*) of dorsal skin and lateral line skin and the yellowness (b*) of abdominal skin showed significantly quadratic pattern with the increase of supplemental BA level (P < 0.05), peaking at group BA600. In term of muscle texture, springiness showed a decreasing trend in significantly linear pattern with the increase of supplemental BA level, bottoming at group BA600. However, cohesiveness and gumminess were significantly linear increased with the increase of supplemental BA level (P < 0.05). Total proportion of n-3LCPUFA showed a linearly increasing trend with the increase of supplemental bile acids level. Muscle TG content was linearly increased with the increase of supplemental bile acids level (P < 0.05), and significant differences occurred as compared to fish fed diets with SO when supplemental bile acids level were reached 600 mg/kg and 1200 mg/kg (P < 0.05). The gene expression of aco was significantly decreased in fish fed diets supplemented with 600 mg/kg bile acids compared with 300 mg/kg other than those fed diets with SO. In conclusion, these results suggested that mixed bile acids supplemented to SO-based diets could improve feed efficiency, skin color and flesh quality to some extent.