AUTHOR=Niu Mingming , Li Xudong , Chen Yuhao , Qin Kangxiang , Liang Guoling , Hu Yun , Jiang Xiaosong , Wang Huan , Zhu Ritong , Wang Chunlin , Mu Changkao 

TITLE=Response of intestinal microbiota to saline-alkaline water in mud crab (Scylla paramamosain) based on multiple low salinity culture modes

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 10 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2023.1153326

DOI=10.3389/fmars.2023.1153326

ISSN=2296-7745

ABSTRACT=The intestinal microbiota acts as an additional "organ" that performs a variety of functions for the host's health. However, the composition and role of the intestinal microbiota in Scylla paramamosain cultivated in inland low salinity saline-alkaline water are unknown. Accordingly, we explored the intestinal microbiota communities and the critical bacteria of S. paramamosain in varieties of multiple low salinity patterns. There were significant differences in the diversity composition of intestinal microbiota and the relative abundance of dominant taxa in each group of cultured crabs. Firmicutes, Proteobacteria, Bacteroidota and Campilobacterota were shown to be the major phyla shared by the four groups, with Bacteroidota having the highest relative abundance (27.10%) in the IS group. Fusobacteriota had the highest proportion in IS group compared with other low salinity groups. A total of 284 indicator bacteria were identified, belonging to eight phyla, and their relative abundances were varied significantly (P < 0.05). Genus Carboxylicivirga, as the indicator bacterium of the IS group, may play a critical role in the adaptation of crab to saline-alkaline water environment. Moreover, salinity may exert considerable selective pressure on the entire microbial community. These findings revealed the features of the intestinal microbiome in S. paramamosain in multiple low salinity patterns, and provided candidate probiotics and basic information for the crab farming in saline-alkaline water, which was conducive to the development and perfection of mud crab breeding technology in inland low salinity saline-alkaline water.