AUTHOR=Hoseinifar Seyed Hossein , Hoseini Seyyed Morteza , Taheri Mirghaed Ali , Ghelichpour Melika , Shirzad-Aski Hesamaddin , Van Doan Hien , El-Haroun Ehab , Safari Roghieh , Khanzadeh Majid 

TITLE=Comparison of the effects of host-associated (autochthonous) and commercial probiotics on immune responses, growth parameters and intestinal microbiota of Caspian whitefish (Rutilus frisii kutum) fry

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 11 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2024.1446927

DOI=10.3389/fmars.2024.1446927

ISSN=2296-7745

ABSTRACT=This study investigates the effects of host-associated probiotic (HAP) vs commercial probiotic (CP) on the growth performance, antioxidant defence and immunity of Caspian whitefish fry. Three hundred whitefish fry (1.15 ± 0.03 g) were randomly divided into five treatments in triplicate. Treatments included feeding with the control group (zero), P. acidilactici as a commercial probiotic (CP) at 6 × 10 8 CFU g -1 , and HA Pediococcus pentosaceus at 10 6 , 10 7 and 10 8 CFU g -1 for eight weeks. Dietary HAP and CP did not have significant effects on growth indices compared to the control group (P > 0.05). However, HAP at 10 7 and 10 8 CFU g -1 and CP significantly increased protein in whitefish carcasses compared to the control group (P < 0.05).Different levels of HAP and CP had a significant effect on whole-body extract (WBE) lysozyme (LZM) activity (P < 0.05). HAP treatment significantly increased WBE ACH50 and bactericidal activity compared to the control and CP group (P < 0.05). Also, in the case of mucosal immune response, different levels of HAP could significantly increase LZM, total immunoglobulin (Ig),agglutination titer, protease and alkaline phosphatase activity compared to the control group (P < 0.05). Whitefish fed HAP showed a significant increase in the activity of WBE antioxidant  parameters (SOD, CAT and GPx) compared to the control group (P < 0.05). Also, feeding withHAP could significantly increase autochthonous LAB levels compared control group (P < 0.05); while the total count of intestinal heterotrophic bacteria was not affected (P > 0.05). Overall, the present study showed HA Pediococcus pentosaceus can be considered as beneficial feed additive for whitefish.