AUTHOR=Xia Yu , Yin Xiaofei , Liu Xiaohui , Xu Mengxue , Weng Deqing , Liu Qian , Chi Wendan , Wu Haiyi 

TITLE=One-year assessment and predictive modeling of macrobenthic communities under thermal discharge and environmental influences near the preoperational YanTai HY-Nuclear Power Plant in the Yellow Sea

JOURNAL=Frontiers in Marine Science

VOLUME=Volume 12 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2025.1637335

DOI=10.3389/fmars.2025.1637335

ISSN=2296-7745

ABSTRACT=The YanTai HY-Nuclear Power Plant (HYNPP) is a newly constructed nuclear power plant that entered operation after 2021. To establish a preoperational ecological baseline for the HYNPP, this study examined macrobenthic community structure and its relationships with multiple environmental variables using year-round field surveys conducted from 2016 to 2017. Eighty-five species from eight phyla were recorded in total, with winter showing the highest species number and spring exhibiting peak biomass. Species composition displayed pronounced seasonal turnover, with replacement rates exceeding 93% between adjacent seasons. Principal coordinates analysis (PCoA) and hierarchical clustering confirmed significant seasonal variation and the localized aggregation of species near the HYNPP. Diversity indices (S, H′, D′, and J′) varied across seasons and spatial gradients, strongly influenced by sea bottom temperature (SBT), salinity, dissolved oxygen (DO), and nutrient concentrations. Spearman correlation analysis and random forest (RF) modeling revealed SBT, DO, phosphate, and phytoplankton cell as dominant factors shaping macrobenthic diversity. RF models provided key insights into nonlinear interactions and variable importance across seasons. Leveraging the dependence-preserving power of copulas, Copula-Based Random Forest (CBRF) models were further developed under a +4°C warming scenario to simulate post-operational thermal-discharge effects; the CBRF framework captured complex spatial responses, predicting localized biomass increases in sheltered muddy areas and biomass reductions in the outer bay. Mollusk biomass was projected to peak in spring near mixed-substrate habitats, while annelids and arthropods showed variable responses linked to sediment type and nutrient availability. These findings highlight strong spatiotemporal coupling between environmental parameters and macrobenthic assemblages, emphasizing the roles of SBT and phytoplankton-driven organic inputs in modulating community structure. The predictive framework built here supports long-term ecological risk assessments and management strategies for mitigating thermal discharge impacts in the Yellow Sea region.