AUTHOR=Cortés-Ortiz Emma , Olayo-Valles R. , Rodríguez-Talavera Rogelio , González-Torres Maykel , Vargas-Muñoz Susana , Olayo R. , Godinez-Fernández R. , Uribe Juárez Omar Eduardo , Morales-Corona Juan 

TITLE=Plasma Functionalized Scaffolds of Polyhydroxybutyrate Electrospun Fibers for Pancreatic Beta Cell Cultures

JOURNAL=Frontiers in Materials

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/materials/articles/10.3389/fmats.2021.600738

DOI=10.3389/fmats.2021.600738

ISSN=2296-8016

ABSTRACT=Polyhydroxybutyrate (PHB) scaffolds, produced by electrospinning and coated by plasma polymerization of pyrrole, were used as cell culture substrate for beta cells. PHB is a natural polymer that is biocompatible and bioresorbable. The electrospinning conditions used, produced scaffolds with large surface area and high porosity. The scaffold surface was modified by plasma polymerization of pyrrole doped with iodine (PPyI). The morphology of the scaffolds was characterized by scanning electron microscopy, while FTIR and Raman spectroscopy were used to characterize the composition. Crystallinity of the PHB fibers was characterized by X-ray diffraction and differential scanning calorimetry. Pancreatic beta cells (from the RIN-M cell line) were cultured on PHB and PHB-PPyI scaffolds. Cell viability results showed that the surface modified material is a good cell culture substrate for beta cells.