AUTHOR=Stoikou Maria , van Breda Shane V. , Schäfer Günther , Vokalova Lenka , Giaglis Stavros , Plattner Alexandra , Infanti Laura , Holbro Andreas , Hahn Sinuhe , Rossi Simona W. , Buser Andreas 

TITLE=G-CSF Infusion for Stem Cell Mobilization Transiently Increases Serum Cell-Free DNA and Protease Concentrations

JOURNAL=Frontiers in Medicine

VOLUME=Volume 7 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2020.00155

DOI=10.3389/fmed.2020.00155

ISSN=2296-858X

ABSTRACT=G-CSF for stem cell mobilisation increases circulating levels of myeloid cells at different stages of maturation. Polymorphonuclear cells (PMNs) are also mobilised in high numbers. It was previously reported that G-CSF primes PMNs towards the release of neutrophils extracellular traps (NETs). Since NETs are often involved in thrombotic events, we hypothesised that high G-CSF blood concentrations could enhance PMN priming towards NET formation in healthy hematopoietic stem cell donors, predisposing them to thrombotic events. However, we found that G-CSF does not prime PMNs toward NETs formation, but increases the serum concentration of cell-free DNA, proteases like neutrophils elastase and myeloperoxidase, and reactive oxygen species. This could possibly create an environment disposed to induce thrombotic events in the presence of additional predisposing factors.