AUTHOR=Díaz-De la Cruz Elodia Nataly , Cerrillos-Gutiérrez José Ignacio , García-Sánchez Andrés , Andrade-Sierra Jorge , Cardona-Muñoz Ernesto Germán , Rojas-Campos Enrique , González-Espinoza Eduardo , Miranda-Díaz Alejandra Guillermina 

TITLE=The Alteration of Pro-inflammatory Cytokines and Oxidative Stress Markers at Six-Month Post-living Kidney Donation

JOURNAL=Frontiers in Medicine

VOLUME=Volume 7 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2020.00382

DOI=10.3389/fmed.2020.00382

ISSN=2296-858X

ABSTRACT=Donors have a higher risk of developing chronic kidney disease than the general population. Some
mechanisms mediated by pro-inflammatory cytokines and oxidative stress may be involved as
risk factors. The objective of the study was to evaluate the behavior of pro-inflammatory
cytokines and oxidative stress markers in living renal donors with a 6-month follow-up. A
single prospective cohort was performed in 88 renal donors. At the end of the follow-up, the
levels of lipoperoxides, 6.52±1.12 mM, and 8-isoprostanes, 63.75±13.28 pg/mL, were lower
than before donation, 10.20±3.95 mM (p<0.001) and 67.54±9.64 pg/mL (p=0.026)
respectively. Initial levels of nitric oxide (NO), 356.09±59.38 μM increased at the end of the
follow-up, 467.08±38.74 μM (p<0.001). It was observed in the final determination of donors
decreased activity of antioxidant enzymes superoxide dismutase (SOD), 0.74±0.57 U/L and glutathione peroxidase (GPx), 556.41±80.37 nmol, in comparison with the levels obtained in
the initial determination, 1.05±0.57 U/L (p<0.001) and 827.93±162.78 nmol (p<0.001)
respectively. The pro-inflammatory cytokines, Tumor necrosis factor alpha and interleukin-6 showed no differences at six months after donation. The enzyme oxoguanine glycosylase
(hOGG1) responsible for repairing oxidative damage to DNA, showed a decrease in its
concentration at the end of the study in donor men, 0.40±0.21 ng/mL compared to the initial
levels, 0.55±0.32 ng/mL (p=0.025). The marker, 8-hydroxy-2-deoxyguanosine (8-OHdG)
exhibited an increase in donor men at the final determination 2.28±1.99 ng/mL, compared to
the concentration before donation, 1.72±1.96 ng/mL (p<0.001). We found significant changes
in the markers of the oxidative state with increased NO and 8-OHdG, as well as a significant
decrease in the antioxidant defenses SOD, GPx, and in the DNA repair enzyme in living renal
donors after six months of follow-up.