AUTHOR=Tai He , Jiang Xiao-lin , Song Nan , Xiao Hong-he , Li Yue , Cheng Mei-jia , Yin Xiao-mei , Chen Yi-ran , Yang Guan-lin , Jiang Xiao-yu , Kuang Jin-song , Lan Zhi-ming , Jia Lian-qun 

TITLE=Tanshinone IIA Combined With Cyclosporine A Alleviates Lung Apoptosis Induced by Renal Ischemia-Reperfusion in Obese Rats

JOURNAL=Frontiers in Medicine

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2021.617393

DOI=10.3389/fmed.2021.617393

ISSN=2296-858X

ABSTRACT=ABSTRACT
Acute lung injury (ALI), which is induced by renal ischemia-reperfusion (IR), is one of the significant reasons for acute kidney injury (AKI)-correlational death. Obesity increases the severity and frequency of ALI and AKI. Tanshinone IIA (TIIA) combined with cyclosporine A (CsA) pretreatment was used to alleviate the lung apoptosis induced by renal IR and to determine whether TIIA combined with CsA can attenuate lung apoptosis by modulating mitochondrial function through the PI3K/Akt/Bad pathway in obese rats. Hematoxylin-eosin (HE) staining was used to assess the histology of the lung injury. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) was used to assess apoptosis of the lung. Electron microscopy was used to assess mitochondrial morphology in lung cells. Arterial blood gas and pulmonary function were used to assess the external respiratory function. Mitochondrial function was used to assess the internal respiratory function and mitochondrial dynamics and biogenesis. Western blot (WB) was used to detect the proteins of the PI3K/Akt/Bad pathway. TIIA combined with CsA can attenuate lung apoptosis through modulating mitochondrial function through the PI3K/Akt/Bad pathway in obese rats. At the same time, TIIA combined with CsA can improve the mitochondrial function in obese rats.