AUTHOR=Tian Xinyao , Hu Yan , Liu Yuanxing , Yang Zhe , Xie Haiyang , Zhou Lin , Zheng Shusen 

TITLE=Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

JOURNAL=Frontiers in Medicine

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2021.626948

DOI=10.3389/fmed.2021.626948

ISSN=2296-858X

ABSTRACT=Ischemic preconditioning (IPC) represents an effective intervention to relieve hepatic ischemia-reperfusion injury (IRI). Systematic detection of circRNAs expression revealing the protection effect of IPC still remain to be elucidated. Here, we applied microarray to detect circRNA and mRNA expression in ischemic liver with and without IPC (n = 3 each group). Compared with sham group, there were 39 circRNAs and 432 mRNAs increased, while 38 circRNAs and 254 mRNAs decreased (fold change ≥1.5, P< 0.05) in the group of hepatic IRI group. As the result of IPC intervention, 43 circRNAs and 64 mRNAs were increased, 7 circRNAs and 31 mRNAs were decreased in the IPC group when comparing with IRI. We then identified circRNA_017753 as the most possible target that may closely related to IPC protective signaling and predicted Jade1 as the target related to circRNA_017753. Three possible circRNA-miRNA-mRNA axes were constructed that may play vital role in protective mechanisms in IPC. The study for the first time systematically detect the dysregulated circRNAs and mRNAs in response to hepatic IRI and IPC intervention. Our profile and bioinformatic analysis provide numerous of novel clews on the understanding the pathophysiologic mechanism of IPC protection against hepatic IRI.