AUTHOR=Abdukhakimova Diyora , Dossybayeva Kuanysh , Grechka Anna , Almukhamedova Zhaina , Boltanova Alyona , Kozina Larissa , Nurgaliyeva Kadisha , Hasanova Liliya , Tanko Matthew N. , Poddighe Dimitri 

TITLE=Reliability of the Multiplex CytoBead CeliAK Immunoassay to Assess Anti-tTG IgA for Celiac Disease Screening

JOURNAL=Frontiers in Medicine

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2021.731067

DOI=10.3389/fmed.2021.731067

ISSN=2296-858X

ABSTRACT=Background and objective: The diagnosis of Celiac Disease is first based on the positivity for specific serological markers. The CytoBead CeliAK immunoassay simultaneously measures antibodies (IgA) directed to anti-tissue transglutaminase (tTG), anti-endomysium (EMA), and deamidated gliadin (DG), in addition to provide a control for normal total IgA levels. The aim of this study is to assess the reliability of this multiplex assay to detect anti-tTG antibody positive patients, compared to a conventional single-parameter ELISA. 
Methods: Serum samples from 149 pediatric patients were assessed by both CytoBead CeliAK immunoassay and ELISA, in order to evaluate their concordance for the measurement of anti-tTG IgA. 
Results: The measurement of anti-tTG IgA by CytoBead CeliAK immunoassay basically showed a concordance rate of 100% between the conventional and single-parameter ELISA, according to the respective cut-off values (3 U/ml and 10 U/ml). 
Conclusions: Our comparative analysis first demonstrates a substantial equivalency between multiplex CytoBead CeliAK assay and the single-parameter conventional ELISA to assess the anti-tTG IgA antibody in the context of the screening for CD. Importantly, CytoBead CeliAK assay presents some pre-analytic, analytic, and economic advantages.