AUTHOR=Zhong Rui , Wu Xiaodong , Liu Zhijuan , He Zeng , Zhang Xuejun , Liu Jiaxin , Cao Ye , Wang Hong 

TITLE=A study on the correlation between hemoglobin concentration and the storage quality of suspended red blood cells prepared from the whole blood of Tibetan male residents

JOURNAL=Frontiers in Medicine

VOLUME=Volume 9 - 2022

YEAR=2023

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2022.1062778

DOI=10.3389/fmed.2022.1062778

ISSN=2296-858X

ABSTRACT=Background: Hypoxia caused hemoglobin (Hb) and hematocrit (Hct) levels to increase and people live at different altitude may have different Hb levels. 
Objectives: In this study, in vitro quality of suspended red blood cells (SRBCs) prepared from whole blood (WB) of Tibetan residents with different Hb concentration([Hb]) throughout storage was measured, and the relationship among major factors in RBC storage with [Hb] was studied. 
Materials and Methods: WB of male Tibetans was divided into three groups according to different [Hb] (group A: 120＜Hb≤185 g/L; group B: 185＜Hb≤210 g/L; group C: Hb>210 g/L). The SRBCs processed by WB were examined aseptically on day 1, 14, 21, and 35. 
Results: Mean corpuscular volume (MCV), adenosine triphosphate (ATP), pH, P50 and hemolysis were no correlation with [Hb]. There was a moderate or high negative association between PLT and [Hb] from day 1 to day 35, and group C was lower than group A during storage. Group C had higher change rates of electrolytes, glucose and lactate, and moderate or high positive correlations between lactate and [Hb] (r = 0.3772, p =0.0045), glucose and [Hb] (r =0.5845, p < 0.0001), Na+ and [Hb] (r =0.3966, p= 0.0027) and K+ between [Hb] (r =0.4885, p =0.0002) were found. Group B had higher 2,3-DPG on day 35, and negative correlation between 2,3-DPG and [Hb] (r = -0.4933, p = 0.0001) was found. 
Conclusions: These new data showed the [Hb] may have implications to the Tibetan SRBCs storage quality, especially on erythrocyte metabolism.