AUTHOR=Miao Sen , Ju Gaoda , Jiang Chonghua , Xue Bing , Zhao Lihua , Zhang Rui , Diao Han , Yu Xingzhou , Zhang Linlin , Pan Xiaozao , Zhang Hua , Zang Lijuan , Wang Lei , Zhou Tianhao 

TITLE=Identification of DYNLT1 associated with proliferation, relapse, and metastasis in breast cancer

JOURNAL=Frontiers in Medicine

VOLUME=Volume 10 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2023.1167676

DOI=10.3389/fmed.2023.1167676

ISSN=2296-858X

ABSTRACT=Background
Breast cancer (BC) is the most common malignant disease in worldwide. Although the survival rate is improved recent years, the prognosis is still bleak once recurrence and metastasis occur. It is vital to investigate more efficiency biomarkers for predicting the metastasis and relapse of BC. DYNLT1 has been reported that participated in the progression of multiple cancers. However, it is still a lack of study about the correlation between DYNLT1 and BC. 
Methods
In this study, we evaluated and validated the expression pattern and prognostic implication of DYNLT1 in BC with multiple public cohorts and BC tumor microarrays (TMAs) of paraffin-embedded tissues collected from Affiliated Hospital of Jining Medical University. The response biomarkers for immune therapy, such as tumor mutational burden (TMB) etc., between different DYNLT1 expression level  BC samples were investigated using data from TCGA-BRCA cohort utilizing public online tools. In addition, colony formation and Transwell assay were conducted to verify the effects of DYNLT1 in BC cell lines proliferation and invasion. 
Results
The results demonstrated that DYNLT1 overexpressed in BC and predicted poor relapse free survival in our own BC TMA cohort. As well, DYNLT1 induced BC development by promoting MDA-MB-231 cells proliferation migration, and metastasis. 
Conclusion
Altogether, our finding proposed that DYNLT1 could be a diagnostic and prognostic indicator in BC.