AUTHOR=Zhu Jiahui , Dai Yijie , Tang Bo , Zhang Hao 

TITLE=The association between serum heat shock protein 72 and intestinal permeability with intestinal microbiota and clinical severity in patients with cerebral infarction

JOURNAL=Frontiers in Medicine

VOLUME=Volume 10 - 2023

YEAR=2024

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2023.1302460

DOI=10.3389/fmed.2023.1302460

ISSN=2296-858X

ABSTRACT=Objectives We aimed to compare serum heat shock protein 72 (HSP72) and intestinal permeability in patients with cerebral infarction (CI) and healthy individuals to reveal their correlations and link to gut microbiota alterations and clinical severity of CI. Methods and Results Stool samples of 50 CI patients and 46 healthy volunteers were applied to characterize intestinal flora profile through 16S rRNA gene sequencing. Serum HSP72 and zonulin were assayed using enzyme-linked immunoassay (ELISA). The obtained data were then subjected to comparative and correlative analysis. We found that the levels of zonulin and serum HSP72 were significantly higher in CI group compared to the healthy group. Serum HSP72 and zonulin levels were positively correlated in the CI group and were correlated positively with the clinical severity of CI. β diversity showed significant differences in intestinal microbiota composition between the two groups. In the CI patient group, the abundance of bacteria Eubacterium_fissicatena_group, Eubacterium_eligens_group and Romboutsia manifested remarkably positive correlation with serum HSP72.The abundance of bacteria Eubacterium_fissicatena_group and Acetivibrio were significantly positive correlation with zonulin levels. Conclusions Our findings represented that an increase in serum HSP72 and zonulin levels were manifested in patients with CI and related to specific gut microbiota alterations and the clinical severity of CI.