AUTHOR=Yuenyong Sumeth , Boonsakan Paisarn , Sripodok Supasan , Thuwajit Peti , Charngkaew Komgrid , Pongpaibul Ananya , Angkathunyakul Napat , Hnoohom Narit , Thuwajit Chanitra 

TITLE=Detection of centroblast cells in H&E stained whole slide image based on object detection

JOURNAL=Frontiers in Medicine

VOLUME=Volume 11 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2024.1303982

DOI=10.3389/fmed.2024.1303982

ISSN=2296-858X

ABSTRACT=Detection and counting of Centroblast cells (CB) in hematoxylin &eosin (H&E) stained whole slide image (WSI) is an important workflow in grading Lymphoma. Each high power field (HPF) patch of a WSI is inspected for the number of CB cells and compared with the World Health Organization (WHO) guideline that organizes lymphoma into 3 grades. Spotting and counting CBs is time-consuming and labor intensive. Moreover, there is often disagreement between different readers, and even a single reader may not be able to perform consistently due to many factors. In this work, we propose an artificial intelligence system that can scan patches from a WSI and detect CBs automatically. The AI system works on the principle of object detection, where the CB is the single class of object of interest. We trained the AI model on 1669 example instances of CBs that originate from WSI of 5 different patients. The data was split 80%/20% for training and validation respectively. The best performance was from YOLOv5x6 model that used the preprocessed CB dataset achieved precision of 0.808, recall of 0.776, mAP at 0.5 IoU of 0.800 and overall mAP of 0.647.