AUTHOR=Li Cuili , Zhong Xi , Guo Shuxian , Liu Feifei , Song Yanhui , Zhao Zhiying , Jiang Guan 

TITLE=A case report of rapid diagnosis of Sporothrix globosa infection using MetaCAP

JOURNAL=Frontiers in Medicine

VOLUME=Volume 12 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2025.1644400

DOI=10.3389/fmed.2025.1644400

ISSN=2296-858X

ABSTRACT=BackgroundSporothrix globosa (S. globosa) is a significant pathogenic fungus responsible for, causing sporotrichosis. Metagenomics capture (MetaCAP), a high-throughput sequencing technology for pathogen nucleic acid detection based on probe capture, facilitates early diagnosis of S. globosa infections. Herein, we present a case of S. globosa infection diagnosed using MetaCAP.Case summaryA 47 year-old female, initially diagnosed with reactive perforating collagenosis, developed epidermal erosion at the affected site after self-applying a poorly air-permeable topical patch purchased online. She subsequently exhibited progressive redness, edema, severe pruritus, and an increase in papules that coalesced into plaques following exposure to decaying wood. She revisited our hospital for further consultation. The diagnosis of sporotrichosis caused by S. globosa was confirmed through a pathological examination of the affected skin tissue and MetaCAP testing. Then, she was treated with itraconazole and naftifine hydrochloride and ketoconazole cream. After a three-month follow-up, the patient’s skin rash showed significant improvement.ConclusionA case of S. globosa infection was promptly diagnosed through MetaCAP and effectively treated with itraconazole.