AUTHOR=Thoma Lina , Sepulveda Edgardo , Latus Annette , Muth Günther TITLE=The stability region of the Streptomyces lividans plasmid pIJ101 encodes a DNA-binding protein recognizing a highly conserved short palindromic sequence motif JOURNAL=Frontiers in Microbiology VOLUME=Volume 5 - 2014 YEAR=2014 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00499 DOI=10.3389/fmicb.2014.00499 ISSN=1664-302X ABSTRACT=
Conjugation is a driving force in the evolution and shaping of bacterial genomes. In antibiotic producing streptomycetes even small plasmids replicating via the rolling-circle mechanism are conjugative. Although they encode only genes involved in replication and transfer, the molecular function of most plasmid encoded proteins is unknown. In this work we show that the conjugative plasmid pIJ101 encodes an overlooked protein, SpdA2. We show that SpdA2 is a DNA binding protein which specifically recognizes a palindromic DNA sequence (