AUTHOR=Zurfluh Katrin , Jakobi Gianna , Stephan Roger , Hächler Herbert , Nüesch-Inderbinen Magdalena 

TITLE=Replicon typing of plasmids carrying blaCTX-M-1 in Enterobacteriaceae of animal, environmental and human origin

JOURNAL=Frontiers in Microbiology

VOLUME=5

YEAR=2014

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00555

DOI=10.3389/fmicb.2014.00555

ISSN=1664-302X

ABSTRACT=<p><bold>Objectives</bold>: The aim of this work was to determine the plasmid replicon profiles of a collection of <italic>bla</italic><sub>CTX-M-1</sub>-positive enterobacterial strains. The isolates originated from chicken in the production pyramid, healthy food-producing animals at slaughter (chicken, calves, and pigs), chicken retail meat, environmental isolates originating from water bodies, and isolates from humans. A selection of IncI and IncN plasmids were characterized by multilocus sequence typing in order to determine their epidemiological relatedness.</p><p><bold>Methods</bold>: Transconjugants of 74 <italic>bla</italic><sub>CTX-M-1</sub>-positive isolates were analyzed by PCR-based replicon typing and by PCR-based plasmid multilocus sequence typing.</p><p><bold>Results</bold>: The incompatibility groups detected among the <italic>bla</italic><sub>CTX-M-1</sub>-harboring plasmids included IncI1, IncN, IncHI1B, IncF, IncFIIS, IncFIB, and IncB/O, with plasmid lineage IncI1/ST3 predominating in isolates from chicken and from humans. Lineage IncN/ST1 was detected mainly in isolates from pigs. For the first time, <italic>bla</italic><sub>CTX-M-1</sub> genes encoded on IncHI1 plasmids were detected in isolates from cattle and from water bodies.</p><p><bold>Conclusions</bold>: This study identifies plasmid lineages that are contributing to the dissemination of <italic>bla</italic><sub>CTX-M-1</sub> genes in the food chain, the environment, and humans.</p>