AUTHOR=Rajeswari Pandiyan , Jose Polpass Arul , Amiya Richa , Jebakumar Solomon Robinson David 

TITLE=Characterization of saltern based Streptomyces sp. and statistical media optimization for its improved antibacterial activity

JOURNAL=Frontiers in Microbiology

VOLUME=5

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00753

DOI=10.3389/fmicb.2014.00753

ISSN=1664-302X

ABSTRACT=<p>A moderately halotolerant <italic>Streptomyces</italic> strain, designated JAJ13 was characterized and a culture medium was statistically optimized to improve its antibacterial activity. Based on the phenotypic and molecular characteristics, strain JAJ13 was identified as a moderately halotolerant <italic>Streptomyces</italic> sp. JAJ13. Novelty of the strain JAJ13 in production of antibacterial compound was assessed by sequence analysis of KSα gene and LC-MS analysis of the active compound. Optimization of the culture medium for antibacterial compound production by the strain JAJ13 was performed with statistical methodology based on experimental designs. Initially, a starch based basal production medium was selected out of eight different production media screened for antibacterial compound production by <italic>Streptomyces</italic> sp. JAJ13. Plackett-Burman design was employed to screen the influential media components affecting the antibacterial compound production. Subsequently, statistical optimization of selected medium components was performed by employing the response surface methodology (RSM) with Box-Behnken design. The optimum initial level of CuSO<sub>4</sub>.5H<sub>2</sub>O, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> and K<sub>2</sub>HPO<sub>4</sub> for the highest antibacterial activity was determined to be at 4.45 mg, 1.96 g, and 1.15 g in 1 L of distilled H<sub>2</sub>O, respectively. PBD and RSM guided design of experiments resulted in a maximum antibacterial activity of 23.37 ± 2.08 mm, which is a 78.8% increase in comparison with that obtained in the unoptimized medium. This study points the success of statistical model in developing an optimized production media for enhanced antibacterial compound production by <italic>Streptomyces</italic> sp. JAJ13.</p>