AUTHOR=Pradel Nathalie , Etienne-Mesmin Lucie , Thévenot Jonathan , Cordonnier Charlotte , Blanquet-Diot Stéphanie , Livrelli Valérie 

TITLE=In vitro adhesion properties of Shiga toxin-producing Escherichia coli isolated from cattle, food, and humans

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 6 - 2015

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00156

DOI=10.3389/fmicb.2015.00156

ISSN=1664-302X

ABSTRACT=Shiga-toxin producing Escherichia coli (STEC) are able to cause serious illnesses ranging from diarrhea to hemorrhagic colitis and hemolytic-uremic syndrome (HUS). These bacteria colonize the digestive tract of humans and produce Shiga-toxins, which are considered to be essential for virulence and are crucial in lethal infection. Colon colonization is supposed to be a determinant step in the development of the infection, but the virulence traits that mediate this step are unclear. We analysed the ability of 256 STEC strains belonging to seropathotype A (the most virulent O157:H7 serotype) to seropathotype E (not involved in human disease) to adhere to HEp-2, HCT-8 and T84 cell lines. The adhesion levels were globally low, but the highest levels were associated to O26:H11 and O103:H2 strains of seropathotype B (associated with outbreaks and HUS but less commonly than serotype O157:H7), possessing both the eae and toxB genes.