AUTHOR=Khieu Thi-Nhan , Liu Min-Jiao , Nimaichand Salam , Quach Ngoc-Tung , Chu-Ky Son , Phi Quyet-Tien , Vu Thu-Trang , Nguyen Tien-Dat , Xiong Zhi , Prabhu Deene M. , Li Wen-Jun 

TITLE=Characterization and evaluation of antimicrobial and cytotoxic effects of Streptomyces sp. HUST012 isolated from medicinal plant Dracaena cochinchinensis Lour.

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00574

DOI=10.3389/fmicb.2015.00574

ISSN=1664-302X

ABSTRACT=<p>A highly potent secondary metabolite producing endophytic strain, <italic>Streptomyces</italic> sp. HUST012 was isolated from the stems of the medicinal plant <italic>Dracaena cochinchinensis</italic> Lour. Strain HUST012 showed antimicrobial and antitumor activities which were significantly much higher than those of dragon's blood extracted from <italic>D. cochinchinensis</italic> Lour. On further analysis, the strain was found to produce two metabolites, SPE-B11.8 (elucidated to be a novel metabolite (<italic>Z</italic>)-tridec-7-ene-1,2,13-tricarboxylic acid) and SPE-B5.4 (elucidated as Actinomycin-D). The Minimum Inhibitory Concentration values of SPE-B11.8 against a set of test bacterial organisms (Methicillin-resistant <italic>Staphylococcus epidermis</italic> ATCC 35984, Methicillin-resistant <italic>Staphylococcus aureus</italic> ATCC 25923, <italic>Escherichia coli</italic> ATCC 25922, and <italic>Klebsiella pneumoniae</italic> ATCC 13883) ranged between 15.63 and 62.5 μg/ml while that for SPE-B5.4 ranged between 0.04 and 2.24 μg/ml. The compound SPE-B11.8 showed cytotoxic effect at 41.63 and 29.54 μg/ml I<italic>C</italic><sub>50</sub>-values against Hep G2 and MCF-7, respectively, while the compound SPE-B5.4 exhibited stronger activities against them at 0.23 and 0.18 μg/ml I<italic>C</italic><sub>50</sub>-values.</p>