AUTHOR=Farshadzadeh Zahra , Hashemi Farhad B. , Rahimi Sara , Pourakbari Babak , Esmaeili Davoud , Haghighi Mohammad A. , Majidpour Ali , Shojaa Saeed , Rahmani Maryam , Gharesi Samira , Aziemzadeh Masoud , Bahador Abbas 

TITLE=Wide distribution of carbapenem resistant Acinetobacter baumannii in burns patients in Iran

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 6 - 2015

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.01146

DOI=10.3389/fmicb.2015.01146

ISSN=1664-302X

ABSTRACT=Antimicrobial resistance in carbapenem non-susceptible Acinetobacter baumannii (CNSAb) is a major public health concern globally. This study determined the antibiotic resistance and molecular epidemiology of CNSAb isolates from a referral burn center in Tehran, Iran.
Sixty-nine CNSAb isolates were tested for susceptibility to antimicrobial agents using the E-test methodology. Multiple locus variable number tandem repeat analysis (MLVA), Multilocus sequence typing and multiplex PCR were performed. PCR assays tested for ambler classes A, B, and D β-lactamases. Detection of ISAba1, characterization of integrons, and biofilm formation were investigated.
Fifty-three (77%) isolates revealed XDR phenotypes. High prevalence of blaOXA-23-like (88%) and blaPER-1 (54%) were detected. ISAba1 was detected upstream of blaADC, blaOXA-23-like and blaOXA51-like   genes in, 97, 42 and 26% of isolates, respectively. Thirty-one (45%) isolates were assigned to International Clone (IC) variants. MLVA identified 56 distinct types with 6 clusters and 53 singleton genotypes. Forty previously known MLST sequence types forming 5 clonal complexes were identified. The Class 1 integron (class 1 integrons) gene was identified in 84% of the isolates. The most prevalent (33%) cassette combination was aacA4-catB8-aadA1. The IC variants were predominant in the A. baumannii lineage with the ability to form strong biofilms.
The XDR-CNSAb from burned patients