AUTHOR=Merino Susana , Tomás Juan M. 

TITLE=The Aeromonas salmonicida Lipopolysaccharide Core from Different Subspecies: The Unusual subsp. pectinolytica

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00125

DOI=10.3389/fmicb.2016.00125

ISSN=1664-302X

ABSTRACT=<p>Initial hydridization tests using <italic>Aeromonas salmonicida</italic> typical and atypical strains showed the possibility of different lipopolysaccharide (LPS) outer cores among these strains. By chemical structural analysis, LPS-core SDS-PAGE gel migration, and functional and comparative genomics we demonstrated that typical <italic>A. salmonicida</italic> (subsp. <italic>salmonicida</italic>) strains and atypical subsp. <italic>masoucida</italic> and probably <italic>smithia</italic> strains showed the same LPS outer core. <italic>A. salmonicida</italic> subsp. <italic>achromogenes</italic> strains show a similar LPS outer core but lack one of the most external residues (a galactose linked α1-6 to heptose), not affecting the O-antigen LPS linkage. <italic>A. salmonicida</italic> subsp. <italic>pectinolytica</italic> strains show a rather changed LPS outer core, which is identical to the LPS outer core from the majority of the <italic>A. hydrophila</italic> strains studied by genomic analyses. The LPS inner core in all tested <italic>A. salmonicida</italic> strains, typical and atypical, is well-conserved. Furthermore, the LPS inner core seems to be conserved in all the <italic>Aeromonas</italic> (psychrophilic or mesophilic) strains studied by genomic analyses.</p>