AUTHOR=Pan Yu-Shan , Zong Zhi-Yong , Yuan Li , Du Xiang-Dang , Huang Hui , Zhong Xing-Hao , Hu Gong-Zheng 

TITLE=Complete Sequence of pEC012, a Multidrug-Resistant IncI1 ST71 Plasmid Carrying blaCTX-M-65, rmtB, fosA3, floR, and oqxAB in an Avian Escherichia coli ST117 Strain

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.01117

DOI=10.3389/fmicb.2016.01117

ISSN=1664-302X

ABSTRACT=<p>A 139,622-bp IncI1 ST71 conjugative plasmid pEC012 from an avian <italic>Escherichia coli</italic> D-ST117 strain was sequenced, which carried five IS<italic>26</italic>-bracketed resistance modules: IS<italic>26</italic>-<italic>fosA3</italic>-<italic>orf1-orf2-Δorf3</italic>-IS<italic>26</italic>, IS<italic>26-fip</italic>-ΔIS<italic>Ecp1</italic>-<italic>bla</italic><sub>CTX-M-65</sub>-IS<italic>903D</italic>-<italic>iroN</italic>-IS<italic>26</italic>, IS<italic>26-ΔtnpR</italic>-<italic>bla</italic><sub>TEM-1</sub>-<italic>rmtB</italic>-IS<italic>26</italic>, IS<italic>26</italic>-<italic>oqxAB</italic>-IS<italic>26</italic>, and IS<italic>26</italic>-<italic>floR</italic>-<italic>aac(3)-IV</italic>-IS<italic>26</italic>. The backbone of pEC012 was similar to that of several other IncI1 ST71 plasmids: pV408, pM105, and pC271, but these plasmids had different arrangements of multidrug resistance region. In addition, the novel IS<italic>Ec57</italic> element was identified, which is in the IS<italic>21</italic> family. The stepwise emergence of multi-resistance regions demonstrated the accumulation of different resistance determinants through homologous recombination. To the best of our knowledge, this is the first study to identify a multidrug-resistant IncI1 ST71 plasmid carrying <italic>bla</italic><sub>CTX-M-65</sub>, <italic>rmtB</italic>, <italic>fosA3</italic>, <italic>floR</italic>, and <italic>oqxAB</italic> in an avian <italic>E. coli</italic> ST117 strain.</p>