AUTHOR=Guo Mingquan , Feng Chunyan , Ren Jie , Zhuang Xuran , Zhang Yan , Zhu Yongzhang , Dong Ke , He Ping , Guo Xiaokui , Qin Jinhong 

TITLE=A Novel Antimicrobial Endolysin, LysPA26, against Pseudomonas aeruginosa

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.00293

DOI=10.3389/fmicb.2017.00293

ISSN=1664-302X

ABSTRACT=<p>The global increase in multidrug resistant (MDR) bacteria has led to phage therapy being refocused upon. A novel endolysin, LysPA26, containing a lysozyme-like domain, was screened against <italic>Pseudomonas aeruginosa</italic> in this study. It had activity against MDR <italic>P. aeruginosa</italic> without pretreatment with an outer-membrane permeabilizer. LysPA26 could kill up to 4 log units <italic>P. aeruginosa</italic> in 30 min. In addition, temperature and pH effect assays revealed that LysPA26 had good stability over a broad range of pH and temperatures. Moreover, LysPA26 could kill other Gram-negative bacteria, such as <italic>Klebsiella pneumonia, Acinetobacter baumannii</italic> and <italic>Escherichia coli</italic>, but not Gram-positive bacteria. Furthermore, LysPA26 could eliminate <italic>P. aeruginosa</italic> in biofilm formation. Our current results show that LysPA26 is a new and promising antimicrobial agent for the combat of Gram-negative pathogens.</p>