AUTHOR=Shukla Shruti , Ahirwal Laxmi , Bajpai Vivek K. , Huh Yun Suk , Han Young-Kyu 

TITLE=Growth Inhibitory Effects of Adhatoda vasica and Its Potential at Reducing Listeria monocytogenes in Chicken Meat

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 8 - 2017

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.01260

DOI=10.3389/fmicb.2017.01260

ISSN=1664-302X

ABSTRACT=The inhibitory effects of Adhatoda vasica ethanolic leaf extract (AVELE) were examined against Listeria monocytogenes, in order to assess its potential to preserve minimally processed meat products safely. Total phenolic, flavonoid, and alkaloid levels in AVELE were 10.09 ± 4.52 mg of gallic acid equivalents (GAE)/g, 22.43 ± 1.62 mg of quercetin equivalents/g, and 19.43 ± 3.90 mg/g, respectively. AVELE (at 1, 5, 10 or 20%) exhibited considerable antibacterial effects against L. monocytogenes; inhibitory zones of 7.4 to 13.6 mm, MIC of 100 mg/mL (10% formulated solution), reduced cell viability, potassium ion efflux, excess release of 260-nm absorbing materials and extracellular ATP. AVELE was used as a rinse solution (at 5, 10 and 20%) for raw chicken breast meat, and a 20% rinsing solution applied for 60 min significantly inhibited L. monocytogenes on raw chicken breast meat, and when the rinse time was increased to 90 min at the same concentration, L. monocytogenes did not grow in meat samples. L. monocytogenes was reduced to ~3 CFU/g after rinsing with 20% or 10% AVELE solution for 30 min. This work demonstrates the effectiveness of AVELE against L. monocytogenes, and suggests AVELE could be used as an effective antimicrobial marinade and rinse on meat products.