AUTHOR=Kleymenov Denis A. , Gushchin Vladimir A. , Gintsburg Alexander L. , Tkachuk Artem P. 

TITLE=Impact of Aerosol Dust on xMAP Multiplex Detection of Different Class Pathogens

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 8 - 2017

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.02341

DOI=10.3389/fmicb.2017.02341

ISSN=1664-302X

ABSTRACT=Environmental or city-scale bioaerosol surveillance can provide additional value for biodefense and public health. Efficient bioaerosol monitoring should rely on multiplex systems capable of detecting a wide variety of biologically hazardous components potentially present in air (bacteria, viruses, toxins and allergens). xMAP technology from LuminexTM allows multiplex bead-based detection of antigens or nucleic acids, but its use for simultaneous detection of different classes of pathogens (bacteria, virus, toxin) is questionable. Another problem is the detection of pathogens in complex matrices, e.g. in the presence of dust. In the present research, we developed the xMAP multiplex test system aiRDeTeX 1.0, which enables detection of RNA virus (influenza A virus), DNA virus (Adenovirus 6), gram-negative bacteria (Salmonella spp.) and toxin (cholera toxin B) as model organisms of biologically hazardous components potentially present or spreadable in the air. We have extensively studied the effect of matrix solution (PBS, distilled water), environmental dust and ultrasound treatment for monoplex and multiplex detection efficiency of individual targets. All targets were efficiently detectable in PBS and in the presence of dust. Ultrasound does not improve the detection except for bacterial LPS.