AUTHOR=Peng Zhong , Liang Wan , Wang Fei , Xu Zhuofei , Xie Zhihao , Lian Zhenghan , Hua Lin , Zhou Rui , Chen Huanchun , Wu Bin 

TITLE=Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.01408

DOI=10.3389/fmicb.2018.01408

ISSN=1664-302X

ABSTRACT=<p><italic>Pasteurella multocida</italic> is a leading cause of respiratory diseases in many host species. To understand the genetic characteristics of <italic>P. multocida</italic> strains isolated from different host species, we sequenced the genomic DNA of <italic>P. multocida</italic> isolated from pigs and analyzed the genetic characteristics of strains from avian species, bovine species, pigs, and rabbits using whole genome sequence (WGS) data. Our results found that a capsular: lipopolysaccharide (LPS): multilocus sequence typing (MLST) genotype A: L1: ST129 (43.75%) was predominant in avian <italic>P. multocida</italic>; while genotypes B: L2: ST122 (60.00%) and A: L3: ST79 (30.00%) were predominate in bovine <italic>P. multocida</italic>; genotype D: L6: ST50 (37.50%) in porcine <italic>P. multocida</italic>; and genotype A: L3: ST9 (76.47%) in rabbit <italic>P. multocida</italic>. Comparative genomic analysis of <italic>P. multocida</italic> from different host species found that there are no genes in the <italic>P. multocida</italic> genome that are specific to any type of host. Phylogenetic analysis using either whole-genome single nucleotide polymorphisms (SNPs) or the set of SNPs present in all single-copy core genes across genomes showed that <italic>P. multocida</italic> strains with the same LPS genotype and MLST genotype were clustered together, suggesting the combining both the LPS and MLST typing schemes better explained the topology seen in the <italic>P. multocida</italic> phylogeny.</p>