AUTHOR=Du Yinju , Wang Chuanqing , Ye Yulong , Liu Yue , Wang Aimin , Li Yong , Zhou Xiaoying , Pan Hanjian , Zhang Jianmin , Xu Xuebin 

TITLE=Molecular Identification of Multidrug-Resistant Campylobacter Species From Diarrheal Patients and Poultry Meat in Shanghai, China

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.01642

DOI=10.3389/fmicb.2018.01642

ISSN=1664-302X

ABSTRACT=<p>Emerging resistance to the antimicrobial agents of choice for treatment of thermophilic <italic>Campylobacter</italic> infections is becoming a serious threat to public health. In this study, 548 <italic>Campylobacter</italic> (372 <italic>C. jejuni</italic> and 176 <italic>C. coli</italic>) isolates from diarrheal patients and poultry meat were subjected for antibiotic susceptibility analysis to ciprofloxacin, tetracycline, gentamicin, erythromycin and clindamycin. Among them, 151 <italic>Campylobacter</italic> (32 <italic>C. jejuni</italic> and 119 <italic>C. coli</italic>) were identified as multidrug resistant isolates. PFGE analysis was performed on the 151 multidrug resistant isolates to determine their genetic relatedness, and 103 PFGE genotypes were determined. Some isolates from both human and chicken belonged to identical genotypes, indicating these clones might be able to spread between human and chicken. Antibiotic resistant genes of the 151 isolates were identified. The numbers of isolates carried <italic>tet (O)</italic>, <italic>aadE, ermB</italic>, and <italic>aadE-sat4-aphA</italic> were 148 (98%), 89 (58.9%), 31 (20.5%), and 10 (6.6%), respectively. Almost all (<italic>n</italic> = 150, 99.3%) had <italic>gyrA</italic> mutation at codon 86. And the 23s rRNA A2075G point mutation was found in 56 (37.1%) isolates. Gene mutations at the <italic>cmeR-cmeABC</italic> intergenic region may lead to the activation of CmeABC multidrug efflux pump, and in this study novel sequence types of the intergenic region were identified in both <italic>C. jejuni</italic> and <italic>C. coli</italic>. This study determined the genetic prerequisites for antibiotic resistance of multidrug resistant <italic>Campylobacter</italic> isolates from diarrheal patients and poultry meat in Shanghai, China.</p>