AUTHOR=Torres Andrea Carolina , Vannini Verónica , Font Graciela , Saavedra Lucila , Taranto María Pía 

TITLE=Novel Pathway for Corrinoid Compounds Production in Lactobacillus

JOURNAL=Frontiers in Microbiology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02256

DOI=10.3389/fmicb.2018.02256

ISSN=1664-302X

ABSTRACT=<p>Vitamin B<sub>12</sub> or cobalamin is an essential metabolite for humans, which makes it an interesting compound for many research groups that focus in different producer-strains synthesis pathways. In this work, we report the influence of key intermediaries for cobalamin synthesis added to the culture medium in two <italic>Lactobacillus</italic> (<italic>L.</italic>) strains, <italic>L. reuteri</italic> CRL 1098 and <italic>L. coryniformis</italic> CRL 1001. Here, we report that addition of Co<sup>2+</sup> and 5,6-dimethylbenzimidazole increased the corrinoid compounds production in both strains while addition of <sc>L</sc>-threonine increased only the corrinoid compounds production by CRL 1001 strain. Then, we purified and characterized by LC-MS the corrinoid compounds obtained. Physiological studies besides <italic>in silico</italic> analysis revealed that <italic>L. reuteri</italic> CRL 1098 and <italic>L. coryniformis</italic> CRL 1001 follow different pathways for the last steps of the corrinoid compounds synthesis.</p>