AUTHOR=Liu Junjie , Yu Zhenhua , Yao Qin , Sui Yueyu , Shi Yu , Chu Haiyan , Tang Caixian , Franks Ashley E. , Jin Jian , Liu Xiaobing , Wang Guanghua 

TITLE=Biogeographic Distribution Patterns of the Archaeal Communities Across the Black Soil Zone of Northeast China

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 10 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.00023

DOI=10.3389/fmicb.2019.00023

ISSN=1664-302X

ABSTRACT=Although archaea are ubiquitous in various environments, the knowledge gaps still exist regarding the biogeographical distribution of archaeal communities at regional scales in agricultural soils compared with bacteria and fungi. To provide a broader biogeographical context of archaeal diversity, this study quantified the abundance and community composition of archaea across the black soil zone in northeast China using real-time PCR and high-throughput sequencing methods. Archaeal abundance across all soil samples ranged from 4.04 ×107 to 26.18×107 16S rRNA gene copies per gram of dry soil. Several soil factors were positively correlated with the abundance including soil pH, concentrations of total C, N and P, and available K in soil, and soil water content. Approximately 94.2%, 5.7% and 0.3% of archaeal sequences, and 31, 151 and 3 OTUs aligned within the phyla Thaumarchaeota, Euryarchaeota and Crenarchaeota, respectively. Within the phylum of Thaumarchaeota, group 1.1b was a dominating genus accounting for an average of 87% archaeal sequence and phylogenetically classified as Nitrososphaera, a genus of ammonia oxidizing archaea. The response of dominating OTUs to environmental factors differed greatly, suggesting the physiological characteristics of different archaeal members is diversified in the black soils. Although the number of OTUs was not related with any particular soil parameters, the number of OTUs within Thaumarchaeota and Euryarchaeota was marginally related with soil pH. Archaeal community composition differed between samples, and a CCA analysis indicated that soil pH and the latitude of sampling locations were two dominating factors in shifting community structure. A VPA analysis showed that the selected soil parameters (32%) were the largest drivers of community variation, in particular soil pH (21%), followed by geographic distances (19%). These findings suggest that archaeal communities have distinct biogeographic distribution pattern in the black soil zone and soil pH was the key edaphic factor in structuring the community compositions.