AUTHOR=Li Hua , Dong Wei , Liu Yong , Zhang Haiyan , Wang Gang 

TITLE=Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 10 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01254

DOI=10.3389/fmicb.2019.01254

ISSN=1664-302X

ABSTRACT=3 nickel-binding peptides were screened from a phage peptide library and displayed separately or in combination on surface-engineered Saccharomyces cerevisiae EBY100. The sorption of nickel ions on the surface of yeast cells increased with the increasing number of nickel Ni(II)-binding peptides displayed. The combined expression of the three peptides by EBY100/pYD1-N123 demonstrated the highest sorption of Ni(II) (2.603±0.004 g g-1, dry weight) and an enhanced sorption capacity of 60.15 %, compared to S. cerevisiae EBY100. An orthogonal test for yeast immobilization was designed. A maximum sorption capability of 68.62 % was observed for a treatment at 25°C with 2.0 % calcium chloride and 3.0 % sodium alginate.