AUTHOR=Malik Yashpal Singh , Verma Atul Kumar , Kumar Naveen , Touil Nadia , Karthik Kumaragurubaran , Tiwari Ruchi , Bora Durlav Prasad , Dhama Kuldeep , Ghosh Souvik , Hemida Maged Gomaa , Abdel-Moneim Ahmed S. , Bányai Krisztián , Vlasova Anastasia N. , Kobayashi Nobumichi , Singh Raj Kumar 

TITLE=Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 10 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01957

DOI=10.3389/fmicb.2019.01957

ISSN=1664-302X

ABSTRACT=The applications of correct diagnostic approaches play a decisive role in timely containment of infectious diseases spread and mitigation of public health risks. Nevertheless, there is a need to update the diagnostics regularly to capture the new, emergent, and highly divergent viruses. Acute gastroenteritis of viral origin has been identified as a significant cause of mortality across the globe, with the more serious consequences seen at the extremes of age groups (young and elderly) and immune-compromised individuals. Therefore, significant advancements and efforts have been put in the development of enteric virus diagnostics to meet the WHO ASSURED criteria as a benchmark over the years. The Enzyme-Linked Immunosorbent (ELISA) and Polymerase Chain Reaction (PCR) are the basic assays that provided the platform for the development of several efficient diagnostics such as real-time RT-PCR, loop-mediated isothermal amplification (LAMP), polymerase spiral reaction (PSR), biosensors, microarrays, and next-generation sequencing. Herein, we describe and discuss the applications of these advanced technologies in context to enteric virus detection by delineating their features, advantages, and limitations.