AUTHOR=Zou Yang , Sun Ye , Qi He , Liu Defeng , Tian Han , Wang Nan , Li Xinming 

TITLE=Streptococcus xiaochunlingii sp. nov. E24 Isolated From the Oropharynx of Healthy Chinese Children

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.563213

DOI=10.3389/fmicb.2020.563213

ISSN=1664-302X

ABSTRACT=<p>A Gram-positive, α-hemolytic, catalase-negative, facultative anaerobic and non-motile coccus was isolated form throat swabs taken from the oropharynx of healthy children. The genome was shown to be 1.950,659 bp long and contained 42.03 mol% G + C bases with 1,942 protein-coding and 53 RNA coding genes. The results of 16S rRNA gene sequencing strongly suggested that the strain is a member of the <italic>Streptococcus</italic> genus, with 98.04, 98.11, and 97.34% similarities to <italic>Streptococcus australis</italic> ATCC 700641<sup>T</sup>, <italic>Streptococcus rubneri</italic> LMG 27207<sup>T</sup> and <italic>Streptococcus parasanguinis</italic> ATCC 15912<sup>T</sup>, respectively. A sodA gene comparison exhibited a sequence identity of 92.6% with the closest strain <italic>Streptococcus australis</italic> ATCC 700641<sup>T</sup>. <italic>In silico</italic> DNA-DNA hybridization showed a highest DNA similarity value of 52% with <italic>Streptococcus australis</italic> ATCC 700641<sup>T</sup>. Comparing 18 biochemical traits, the similarities of the <italic>Streptococcus</italic> strain E24 were 72% with <italic>Streptococcus rubneri</italic> LMG 27207<sup>T</sup>, 78% with <italic>Streptococcus australis</italic> ATCC 700641<sup>T</sup> and 44% with <italic>Streptococcus parasanguinis</italic> ATCC 15912<sup>T</sup>. We suggest that based on the genotypic and phenotypic results that the strain E24 is a novel species of the <italic>Streptococcus</italic> genus and propose the name <italic>Streptococcus xiaochunlingii</italic> sp. nov. E24.</p>