AUTHOR=Fan Shihui , Ma Chao , Tian Xiaopeng , Ma Xiaoyi , Qin Mingcan , Wu Hangjie , Tian Xueqing , Lu Jing , Lyu Mingsheng , Wang Shujun TITLE=Detection of Vibrio vulnificus in Seafood With a DNAzyme-Based Biosensor JOURNAL=Frontiers in Microbiology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.655845 DOI=10.3389/fmicb.2021.655845 ISSN=1664-302X ABSTRACT=

Vibrio vulnificus is an important pathogenic bacterium that is often associated with seafood-borne illnesses. Therefore, to detect this pathogen in aquatic products, a DNAzyme-based fluorescent sensor was developed for the in vitro detection of V. vulnificus. After screening and mutation, a DNAzyme that we denominated “RFD-VV-M2” exhibited the highest activity, specificity, and sensitivity. The limit of detection was 2.2 × 103 CFU/ml, and results could be obtained within 5–10 min. Our findings suggested that the target of DNAzyme RFD-VV-M2 was a protein with a molecular weight between 50 and 100 kDa. The proposed biosensor exhibited an excellent capacity to detect marine products contaminated with V. vulnificus. Therefore, our study established a rapid, simple, sensitive, and highly specific detection method for V. vulnificus in aquatic products.