AUTHOR=Kim Seunghwan , Jang Wooyoung Eric , Park Jihwan , Kim Min-Sik , Kim Jeong-Gu , Kang Lin-Woo 

TITLE=Combined Analysis of the Time-Resolved Transcriptome and Proteome of Plant Pathogen Xanthomonas oryzae pv. oryzae

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.664857

DOI=10.3389/fmicb.2021.664857

ISSN=1664-302X

ABSTRACT=<p><italic>Xanthomonas oryzae</italic> pv. <italic>oryzae</italic> (<italic>Xoo</italic>) is a plant pathogen responsible for causing bacterial blight in rice. The immediate alterations in <italic>Xoo</italic> upon initial contact with rice are essential for pathogenesis. We studied time-resolved genome-wide gene expression in pathogenicity-activated <italic>Xoo</italic> cells at the transcriptome and proteome levels. The early response genes of <italic>Xoo</italic> include genes related to cell motility, inorganic ion transport, and effectors. The alteration of gene expression is initiated as early as few minutes after the initial interaction and changes with time. The time-resolved comparison of the transcriptome and proteome shows the differences between transcriptional and translational expression peaks in many genes, although the overall expression pattern of mRNAs and proteins is conserved. The discrepancy suggests an important role of translational regulation in <italic>Xoo</italic> at the early stages of pathogenesis. The gene expression analysis using time-resolved transcriptome and proteome provides unprecedented valuable information regarding <italic>Xoo</italic> pathogenesis.</p>