AUTHOR=Huang Ke , Tang Jianming , Zou Yong , Sun Xiangcheng , Lan Jianbin , Wang Wei , Xu Panpan , Wu Xiangwei , Ma Rui , Wang Qi , Wang Zhenshuo , Liu Jia 

TITLE=Whole Genome Sequence of Alternaria alternata, the Causal Agent of Black Spot of Kiwifruit

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.713462

DOI=10.3389/fmicb.2021.713462

ISSN=1664-302X

ABSTRACT=Alternaria alternata is a pathogen in a wide range of agriculture crops, and causes significant economic losses. A strain of A. alternata (Y784-BC03) was isolated and identified from ‘Hongyang’ kiwifruit and demonstrated to cause black spot infections on fruits. The genome sequence of Y784-BC03 was obtained using Nanopore MinION technology. The assembled genome is composed of 33,869,130 bp (32.30 Mb) comprising 10 chromosomes, and 11954 genes. A total of 2180 virulence factors were predicted to be present in the obtained genome sequence. The virulence factors comprised genes encoding secondary metabolites, including host-specific and non-host-specific toxins, cell wall-degrading enzymes, and major transcriptional regulators. The predicted gene clusters encoding genes for the biosynthesis and export of secondary metabolites in the genome of Y784-BC03 were associated with host-specific toxins, including ACR-toxin, and non-host-specific toxins, including cercosporin , dothistromin and versicolorin B. Major transcriptional regulators of different mycotoxin biosynthesis pathways were identified, including the transcriptional regulators, polyketide synthase, P450 monooxygenase, and major facilitator superfamily transporters.